Development and validation of a simple reversed-phase HPLC method for the determination of camptothecin in animal organs following administration in solid lipid nanoparticles

► Development of a HPLC method for camptothecin (CPT) pharmacokinetic studies. ► CPT was administered in solid lipid nanoparticles to rats. ► Fluorescence detection allowed quantification limit value ≤0.5 ng/mL. ► The method was specific, accurate and precise in all biological matrices. ► CPT was stable after 24 h at RT or at 4 °C, or after three freeze/thaw cycles.