کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7619445 1494162 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of puerarin by capillary electrophoresis with chemiluminescence detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of puerarin by capillary electrophoresis with chemiluminescence detection
چکیده انگلیسی
A new analytical method for puerarin using capillary electrophoretic (CE) separation and chemiluminescence (CL) detection has been developed. The detection was based on the enhanced CL intensity of the reaction between luminol and potassium ferricyanide by puerarin in alkaline solution. A laboratory-built CE-CL apparatus was deployed for the puerarin detection. Under the optimal conditions, a linear range from 5.0 × 10−8 to 2.5 × 10−6 M and a detection limit of 1.0 × 10−8 M (S/N = 3) for puerarin were achieved. The determination of puerarin was achieved in less than 5 min, and the proposed method was applied to the determination of puerarin in pharmaceutical, human urine and human plasma samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 877, Issues 14–15, 15 May 2009, Pages 1591-1594
نویسندگان
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