Serum collected from fruit and vegetable juice treated rats antagonizing H2O2-induced oxidative damage in PC12 cells

Epidemiology studies indicate that fruit and vegetable (FV) rich diets are associated with lower risk of Alzheimer's disease (AD), while, the underlying mechanism is not clear. The health promoting effects of diet-derived antioxidants (such as purified vitamin C, β-carotene, flavonoids) were indicated in dietary intervention experiments, However, the practical biological efficiency of FV consumed through diet could not be simply mimicked by purified antioxidants or their mixture. An in vitro neuron cell (PC12) culture experiment was carried out in the present study to observe the oxidative damage preventing effects of serum collected from FV juice treated rats. Our results showed that serum collected from FV juice treated rats antagonized the oxidative damage caused by H2O2 treatment, and regulated the antioxidant gene expression. FV juice increased antioxidant biomarker levels and antioxidant enzyme activity in rat serum. Our results indicate that multiple mechanisms are involved in the neuron protective effects of FV-rich diet.