کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7625068 | 1494554 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quercetin increases claudin-4 expression through multiple transcription factors in intestinal Caco-2 cells
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
We previously reported that quercetin increases tight junction (TJ) barrier integrity in intestinal Caco-2 cells. This study was aimed at determining the molecular mechanism underlying the quercetin-mediated effect with a particular focus on the transcriptional regulation of claudin-4. Quercetin aglycone, but not its glycosides, enhanced TJ barrier integrity of Caco-2 cells, indicated by transepithelial electrical resistance. Immunoblot analysis, qPCR, and luciferase promoter assay revealed that quercetin transcriptionally increased the claudin-4 expression. Narrowing the claudin-4 promoter sequences from 1866âbp to 200âbp did not affect quercetin-mediated promoter activation. Site-directed mutation of SP1, AP-1 and GATA binding sites within the 200âbp sequence partially suppressed the effects of quercetin on claudin-4 promoter activity. Taken together, quercetin enhances the TJ barrier integrity in intestinal Caco-2 cells, with the transcription factors, SP1, AP-1, and GATA, involved in the quercetin-mediated transcriptional regulation of claudin-4.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Functional Foods - Volume 10, September 2014, Pages 112-116
Journal: Journal of Functional Foods - Volume 10, September 2014, Pages 112-116
نویسندگان
Sakino Noda, Soichi Tanabe, Takuya Suzuki,