کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7625649 | 1494576 | 2018 | 21 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
On-line coupling of achiral Reversed Phase Liquid Chromatography and chiral Supercritical Fluid Chromatography for the analysis of pharmaceutical compounds
ترجمه فارسی عنوان
اتصال به اینترنت از طریق کروماتوگرافی مایع فازی آریلال معکوس و کروماتوگرافی مایع کریال سوپر کریتیک برای تجزیه و تحلیل ترکیبات دارویی
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
On-line selective comprehensive two-dimensional chromatography combining Reversed Phase Liquid Chromatography and Supercritical Fluid Chromatography (sRPLCxSFC) was investigated for the analysis of chiral pharmaceutical compounds. Preliminary studies were carried out with the aim of overcoming instrumental constraints which are related to such 2D-coupling. The impact of both injection solvent and injection volume on the chiral SFC second separation was assessed with a view to limiting injection effects due to mobile phase compatibility issues between both dimensions. The resulting on-line sRPLCxSFC system was applied to the achiral x chiral analysis of a pharmaceutical sample. Using an Acquity BEH C18 column in the first dimension and a Chiralpak IC column in the second one, both chemical (achiral) and enantiomeric (chiral) purities could be evaluated in less than 50â¯min within a single run. Under such conditions, a detection limit of about 0.5% for R-enantiomer could be obtained with UV detection. The results obtained in sRPLCxSFC were compared to those obtained in conventional chiral 1D-SFC. Baseline resolution was obtained in both cases and the linearity in the detector response was on the same order of magnitude (R²â¯>â¯0.99). Finally, despite current instrumental limitations (no commercially available system for sLCxSFC, large dwell volume and large extra-column volume in SFC), the on-line coupling of RPLC and SFC appears to be attractive and promising for rapid achiral/chiral analysis of complex pharmaceutical samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 159, 10 September 2018, Pages 237-244
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 159, 10 September 2018, Pages 237-244
نویسندگان
Marion Iguiniz, Estelle Corbel, Nicolas Roques, Sabine Heinisch,