کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7628580 | 1494604 | 2016 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Angiotensin I-converting enzyme (ACE) inhibitory activity of Fucus spiralis macroalgae and influence of the extracts storage temperature-A short report
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Recently, increasing attention has been paid to the marine algae as a natural source of novel angiotensin-I converting enzyme (ACE) inhibitors, such as the phlorotannins that are the predominant polyphenols in brown algae. This study reports, for the first time, the ACE inhibition of methanol extract/fractions from Azorean brown algae Fucus spiralis (Fs) determined by HPLC-UV method, their total phenolic content (TPC) quantified as phloroglucinol equivalents (PE) and the effect of the Fs dry powder methanol extracts (Fs-DME) storage temperature on ACE inhibition. The results indicate that the ACE inhibition of Fs-DME decreased by 28.8% and 78.2% when stored during 15 days at â80 °C and â13 °C, respectively, as compared with the activity of Fs-DME at a refrigerated temperature of 6 °C and assayed immediately after extraction that showed a value of 80.1 ± 2.1%. This Fs-DME sample was fractionated by ultrafiltration membranes into three molecular weight ranges (<1 kDa, 1-3 kDa and >3 kDa), presenting the fraction >3 kDa remarkably high ACE inhibition (88.8 ± 2.4%), TPC value (156.6 ± 1.4 mg PE/g of dry weight fraction) and yield. Furthermore, chromatographic and spectrophotometric analyses corroborate that phenolic compounds were present in Fs methanol extract/fractions, and also revealed that phloroglucinol occurs in Fs. The results seem to suggest that Azorean Fs can be a source of powerful ACE-inhibitory phlorotannins with potential impact on public health, particularly on hypertensive patients.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 131, 30 November 2016, Pages 503-507
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 131, 30 November 2016, Pages 503-507
نویسندگان
Lisete Paiva, Elisabete Lima, Ana Isabel Neto, José Baptista,