کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7628611 | 1494607 | 2016 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bioanalytical method development and validation for the determination of glycine in human cerebrospinal fluid by ion-pair reversed-phase liquid chromatography-tandem mass spectrometry
ترجمه فارسی عنوان
توسعه و اعتبار سنجی روش بیوانیالیتی برای تعیین گلیسین در مایع مغزی نخاعی انسان با استفاده از اسپکترومتر جرمی کروماتوگرافی مایع فاز مایع برگشت پذیر
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کلمات کلیدی
LC-UVLLOQFDAISRMRMLC–MS/MS - LC-MS / MSinternal standard - استاندارد داخلیincurred sample reanalysis - بازجویی نمونه انجام گرفتlower limit of quantification - حد پایین کمیت سنجیFood and Drug Administration - سازمان غذا و داروLiquid chromatography-tandem mass spectrometry - طیف سنجی جرمی کروماتوگرافی مایع دو طرفهStandard addition - علاوه بر استانداردCerebrospinal fluid - مایع مغزی نخاعیCSF - مایع مغزی نخاعیmultiple reaction monitoring - نظارت چندگانه چندگانهParallelism - همبستگیquality control - کنترل کیفیتGlycine - گلیسین
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A LC-MS/MS method has been developed and validated for the determination of glycine in human cerebrospinal fluid (CSF). The validated method used artificial cerebrospinal fluid as a surrogate matrix for calibration standards. The calibration curve range for the assay was 100-10,000 ng/mL and 13C2, 15N-glycine was used as an internal standard (IS). Pre-validation experiments were performed to demonstrate parallelism with surrogate matrix and standard addition methods. The mean endogenous glycine concentration in a pooled human CSF determined on three days by using artificial CSF as a surrogate matrix and the method of standard addition was found to be 748 ± 30.6 and 768 ± 18.1 ng/mL, respectively. A percentage difference of â2.6% indicated that artificial CSF could be used as a surrogate calibration matrix for the determination of glycine in human CSF. Quality control (QC) samples, except the lower limit of quantitation (LLOQ) QC and low QC samples, were prepared by spiking glycine into aliquots of pooled human CSF sample. The low QC sample was prepared from a separate pooled human CSF sample containing low endogenous glycine concentrations, while the LLOQ QC sample was prepared in artificial CSF. Standard addition was used extensively to evaluate matrix effects during validation. The validated method was used to determine the endogenous glycine concentrations in human CSF samples. Incurred sample reanalysis demonstrated reproducibility of the method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 128, 5 September 2016, Pages 132-140
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 128, 5 September 2016, Pages 132-140
نویسندگان
Jian Jiang, Christopher A. James, Philip Wong,