Preparation of a pipette tip-based molecularly imprinted solid-phase microextraction monolith by epitope approach and its application for determination of enkephalins in human cerebrospinal fluid

- A new MIP monolith for enkephalins was prepared by epitope imprinting technique.
- MIP monolith was prepared in a micropipette tip which can been used as SPME column.
- This MIP monolith exhibited highly selective extraction efficiency for enkephalins.
- Quantization of enkephalins in CSF was developed by MISPME coupling to HPLC/UV.