High resolution separation of recombinant monoclonal antibodies by size-exclusion ultra-high performance liquid chromatography (SE-UHPLC)

- SE-UHPLC was evaluated for antibody characterization.
- High back pressures did not generate HMWS under conditions studied.
- The fragment peaks separated by SE-UHPLC were characterized.
- SE-UHPLC provides higher resolution and higher throughput separation.