Formation of GSH-trapped reactive metabolites in human liver microsomes, S9 fraction, HepaRG-cells, and human hepatocytes

- Different enzyme sources were compared on their ability to form GSH conjugates.
- Incubations with microsomes, S9 fraction, hepatocytes and HepaRG cells.
- Test pattern of 10 diverse test compounds.
- Incubations with subfractions produced more GSH-conjugates than cell-based systems.
- Different major GSH conjugates in subfractions and cell-based systems.