Simultaneous determination of propofol and its glucuronide in whole blood by liquid chromatography-electrospray tandem mass spectrometry and the influence of sample storage conditions on the reliability of the test results

- The LC-MS/MS method simultaneously determines propofol and propofol β-d-glucuronide.
- The LC-MS/MS method does not require chemical modifications of the analytes.
- The lower limit of quantification is 0.01 mg/L for propofol in ante-mortem blood.
- The stability of propofol in blood is less at −20 °C compared to 5 °C and −80 °C.
- The stability of propofol depends on the stabilisation mixture used.