Application of an LC-MS/MS method for the simultaneous quantification of human intestinal transporter proteins absolute abundance using a QconCAT technique

- Establishing lower limit of quantification, linearity and precision of the QconCAT SRM assay.
- Describing a calcium chelation method to harvest human enterocytes.
- Utilising a gravimetric method to account for peptide losses in proteolytic digestion.
- Quantifying six transporter protein abundances in human intestinal samples by QconCAT.