Method development and validation of arene substituted regioisomers in a pharmaceutical candidate by high temperature GC-FID

- Separation of non-polar and semi-volatile arene regioisomers without derivatization.
- Utilization of high temperature gas chromatography with flame ionization detection.
- Validation parameters included linearity, precision, accuracy, repeatability and sensitivity.
- Broad range of applications where separation of isomers is required.