Determination of carnitine and acylcarnitines in human urine by means of microextraction in packed sorbent and hydrophilic interaction chromatography-ultra-high-performance liquid chromatography-tandem mass spectrometry

- HILIC-UHPLC-MS/MS method for determination of carnitine and acylcarnitines.
- HILIC separation of carnitine and acylcarnitines was achieved over a 2.5-min.
- Suitability of MEPS technique to isolate potential cardiovascular biomarkers.
- The method validation demonstrated satisfactory analytical performance data.
- The MEPS-HILIC-UHPLC-MS/MS method can be used in clinical studies.