Sensitive and accurate liquid chromatography-tandem mass spectrometry methods for quantitative determination of a novel hepatitis C NS5B inhibitor BMS-791325 and its active metabolite in human plasma and urine

- Quantitation of BMS-791325 and active metabolite in plasma and urine by LC-MS/MS.
- The validation results demonstrate the method is accurate, precise, and reproducible.
- Coadministered famotidine and ritonavir did not affect the LC-MS/MS method.
- Crossvalidation after method transfers demonstrated method equivalency.
- The plasma method was used for pharmacokinetic evaluation in many clinical studies.