Simultaneous quantitative determination of celecoxib and its two metabolites using liquid chromatography-tandem mass spectrometry in alternating polarity switching mode

- Development of quantitative analytical method for celecoxib and its two main metabolites.
- The simultaneous detection of celecoxib, hydroxycelecoxib and celecoxib carboxylic acid.
- Using liquid chromatography-tandem mass spectrometry in alternating polarity switching mode.
- Successful application to the pharmacokinetic study of celecoxib in rat plasma.