Multi-responses extraction optimization combined with high-performance liquid chromatography-diode array detection-electrospray ionization-tandem mass spectrometry and chemometrics techniques for the fingerprint analysis of Aloe barbadensis Miller

- Multi-responses extraction optimization based on RSM was implemented successfully.
- HPLC fingerprint was established for the overall quality control of A. barbadensis.
- 27 common peaks were recognized from 15 batches of samples.
- 25 common peaks were characterized by HPLC-DAD-ESI-MS/MS method.
- Chemometrics techniques were used for classify A. barbadensis samples.