Analytical approach for the determination of steroid profile of humans by gas chromatography isotope ratio mass spectrometry aimed at distinguishing between endogenous and exogenous steroids

- Procedure for determination of steroids by GC/IRMS in urine was validated.
- Improvement of the chromatographic resolution of steroids was achieved.
- The accurate discrimination of endogenous versus exogenous AAS in urine was verified.
- δ13C/12C was used to evaluate suspicious steroid and endogenous reference compound.