Bioassay-guided preparative separation of angiotensin-converting enzyme inhibitory C-flavone glycosides from Desmodium styracifolium by recycling complexation high-speed counter-current chromatography

- Bioactivity-guided fractionation based on HSCCC was applied.
- C-flavone glycosides were obtained by recycling complexation HSCCC.
- The target-isolated compounds showed effective ACE inhibitory activities.
- The hybrid strategy is powerful for discovery of bioactive constituents from TCMs.