Development and validation of a supercritical fluid chromatography method for the direct determination of enantiomeric purity of provitamin B5 in cosmetic formulations with mass spectrometric detection

- Panthenol (provitamin B5) enantiomers are separated in supercritical fluid chromatography.
- Sample preparation was optimized for a cream sample.
- Optical purity of dexpanthenol is measured with electrospray ionization mass spectrometry.
- The proposed method is validated.