Quantitative LC-MS/MS determination of flupirtine, its N-acetylated and two mercapturic acid derivatives in man

- Comprehensive validation of a LC-MS/MS assay for flupirtine, D-13223 in human serum, urine and feces (higher sensitivity).
- First validated LC-MS/MS assay to quantify two mercapturic acid derivates of flupirtine (M-424, M-466) in human urine.
- Application of these two methods to characterize the pharmacokinetics of FLU, D-13223, M-424 and M-466 in humans.