A μ-SPE procedure for the determination of cannabinoids and their metabolites in urine by LC-MS/MS

- Analysis of THC-COOH, THC, THC-OH, CBD and CBN in urine by LC-MS/MS.
- Tandem hydrolysis, enzymatic and basic for glucuronide cleavage.
- Micro-SPE extraction using only 90 μL of urine.
- Time and enzyme reduction: the cost of the analysis is minimized.
- The procedure has been validated according to SWGTOX guidelines.