Improved detection of variants in recombinant human interferon alpha-2a products by reverse-phase high-performance liquid chromatography on a core-shell stationary phase

Chromatograms A and B show improved RP-HPLC separation of N-methionylated variant of rhIFN α-2a drug substance and finished product while chromatograms C and D show the same products under conditions reported in current EP monograph.