Application of a stabilizer cocktail of N-ethylmaleimide and phenylmethanesulfonyl fluoride to concurrently stabilize the disulfide and ester containing compounds in a plasma LC-MS/MS assay

- Quantified an epothilone-folate conjugate and its epothilone moiety in plasma.
- Evaluated the stability of the disulfide and ester containing compounds in plasma.
- A cocktail of NEM and PMSF effectively stabilized both analytes in plasma.
- ISR helps identifying the minor conversion from BMS-753493 to BMS-748285.
- The validated method was successfully applied to support clinical studies.