Sensitive liquid chromatography/mass spectrometry methods for quantification of pomalidomide in mouse plasma and brain tissue

- Pomalidomide is FDA approved for multiple myeloma and is under evaluation for use in other diseases.
- First report of a fully validated LC-MS/MS assay for pomalidomide in mouse plasma and brain.
- First report of pomalidomide plasma and brain pharmacokinetics in mice.
- Sensitive assay with LLOQ of 0.3 nM (0.082 ng/mL) for plasma and 0.6 pmol/g for brain.
- Small amounts of samples are needed for this sensitive method (100 μL plasma or 50 mg brain tissue).