کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7631563 1494650 2013 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
LC-ESI-MS/MS quantification of 4β-hydroxycholesterol and cholesterol in plasma samples of limited volume
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
LC-ESI-MS/MS quantification of 4β-hydroxycholesterol and cholesterol in plasma samples of limited volume
چکیده انگلیسی

- An assay was qualified for quantification of 4β-hydroxycholesterol and cholesterol.
- This assay was based on isotope-dilution, surrogate matrix and LC-ESI-MS/MS.
- The assay used only 5-μL sample and has acceptable sensitivity and reproducibility.
- Assay was validated by incurred sample reanalysis and cholesterol enzymatic method.
- Both analytes were quantified in plasma from human, naïve and FRG chimeric mice.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 85, November 2013, Pages 145-154
نویسندگان
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