Simultaneous monitoring of superoxides and intracellular calcium ions in neutrophils by chemiluminescence and fluorescence: Evaluation of action mechanisms of bioactive compounds in foods

- We developed the method to measure anti-inflammatory and antioxidant activities.
- The method utilizes the innate immune response of neutrophils.
- We validated the method by using known anti-inflammatory agents and antioxidants.
- Our method can be used for evaluating the action of bioactive compounds in food.