Critical evaluation of fast size exclusion chromatographic separations of protein aggregates, applying sub-2 μm particles

► The performance of 1.7, 3 and 5 μm packings were compared in size exclusion chromatography. ► The effect of temperature on separation performance was studied in size exclusion chromatography. ► The effect of ultra-high pressure on protein aggregation was evaluated. ► Sub-3 min size exclusion separations of proteins were performed.