Comparison of supercritical fluid chromatography and reverse phase liquid chromatography for the impurity profiling of the antiretroviral drugs lamivudine/BMS-986001/efavirenz in a combination tablet

► Impurity profiling of a triple combination anti-HIV drug by SFC and RPLC is reported. ► Method development strategies and figures of merit with UV detection are discussed. ► A high degree of orthogonality was achieved with SFC. ► SFC also gave a more uniform distribution of components across the separation space. ► Potential impurities/degradation products were quantitated at the ≤0.1 area% level.