A fast and efficient HPLC-PDA-MS method for detection and identification of pyranochromanone acids in Calophyllum species

► HPLC-PDA-MSn method is a rapid and efficient tool for the analysis of pyranochromanones in Calophyllum spp. ► Leaves and resins of Cuban Calophyllum spp are a rich source of pyranochromanone acids. ► Three new pyranochromanone acids were identified by MSn and NMR analyses.