Determination of neomycin and related substances in pharmaceutical preparations by reversed-phase high performance liquid chromatography with mass spectrometry and charged aerosol detection

► Determination content of neomycin and related substances was reported. ► Charged aerosol detector connected with HPLC system was used. ► The biggest advantage of new method is straightforward quantification of all impurities. ► The elaborate LC-CAD method was applied for analyzing commercial products.