On-line solid-phase extraction with ultra performance liquid chromatography and tandem mass spectrometry for the detection of nicotine, cotinine and trans-3′-hydroxycotinine in urine to strengthen human biomonitoring and smoking cessation studies

► First on-line SPE coupling to UPLC-MS/MS for detection of nicotine and 2 metabolites. ► Fast, sensitive, robust and very cost-effective detection method in urine. ► Short and simple sample preparation without compromising instrumental robustness. ► Ideal for high-throughput detection of very low passive exposure to tobacco smoke.