Development, validation and comparison of two microextraction techniques for the rapid and sensitive determination of pregabalin in urine and pharmaceutical formulations after ethyl chloroformate derivatization followed by gas chromatography-mass spectrom

► For the first time the usage of SPME and DLLME coupled with GC-MS detection for the extraction and analysis of pregabalin. ► Results exhibit good linearity, precision and detection limit for both techniques. ► The developed methods may find wide application for the routine determination of PRG in biological as well as in quality control samples of pharmaceutical formulations.