Simultaneous determination of nine model compounds in permeability samples using RP-HPLC: Application to prove the cassette administration principle in single pass intestinal perfusion study in rats

► To the best of our knowledge, there is no simultaneous analytical method reported for at least two model drugs from each permeability class. ► A simple and reliable RP-HPLC method has been developed and validated for simultaneous determination of nine permeability model drugs. ► Applicability of the cassette dosing principle and its practice to intestinal perfusion studies for the first time.