Proteome analysis of post-transplantation recovery mechanisms of an EAE model of multiple sclerosis treated with embryonic stem cell-derived neural precursors

We have treated a score 3 mouse experimental autoimmune encephalomyelitis (EAE) model of multiple sclerosis (MS) with an embryonic stem cell-derived neural precursor. Clinical analysis showed recovery of the EAE model of MS following transplantation. We analyzed the proteome of spinal cords of healthy and EAE samples before and after transplantation. We identified 72 proteins which showed differentially that spinal cord protein spots changed in the EAE or transplanted mouse compared to controls. Of these, the amounts of 27 differentially expressed proteins in EAE samples returned to sham levels after transplantation, suggesting a possible correlation between changes at the proteome level and clinical signs of EAE in transplanted mice.172