The wheat chloroplastic proteome

The extracted protein fractions from chloroplast and sub-fractions of chloroplasts by ultra-centrifugations were separated by 1D-PAGE and analyzed with LTQ-FTICR mass spectrometry. This enabled us to detect and identify 767 unique proteins. In all, 67% were confirmed as chloroplast thylakoid proteins. Nearly complete protein coverage (89% proteins) has been accomplished for the key chloroplast pathways namely photosynthesis pathway in wheat. Protein abundance within the chloroplasts was examined by two-dimensional electrophoresis under salt and water stress. More than 100 protein spots were reproducibly detected on each gel, 21 protein spots were differentially expressed during salt treatment. Using LTQ-FTICR hybrid mass spectrometry, 65 unique proteins assigned in the differentially abundant spots. While 20 differentially expressed proteins were detected in the chloroplasts and analyzed with high through put MALDI-TOF/TOF mass spectrometry during water stress.262