Systematic characterization of the specificity of the SH2 domains of cytoplasmic tyrosine kinases

► The specificities of 25 CTK SH2 domains are determined by screening OBOC peptide libraries. ► SVM models are created for the 25 SH2 domains for predicting SH2-ligand interactions. ► The specificities of the CTK SH2 domains show less overlap than those of the kinase domains.