Spermogramme et spermocytogramme manuels et automatisés-Test de migration-survie

Conventional semen parameters (spermogram, sperm morphology) are among first line diagnostic tests to perform when facing an infertile couple. Furthermore, if a therapeutic intervention is needed, they must be completed by a sperm preparation technic allowing to witness which assisted reproductive option is possible from a male point of view. All analysis of semen parameters are usually performed using manual technics whose methodology is highly codified but that are known to be submitted to several sources of variability, notably the operator, imposing a regular assessment of technical skills using pluri-annual quality controls. The biologist must be aware of the subjectivity of numerous semen parameters and must know how to cope with to limit its effects. Regarding, sperm morphology, he must know the reference values of the classification he used and can argument on the reasons to choose it, be aware of the lack of clinical evidence to perform a detailed analysis of every possible sperm abnormalities. In this context, the implementation of automatized approaches of standard semen evaluation is probably a positive point, although they also have constraints and limitations, among which their cost, needing a minimal volume of activity to be considered.