کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
76457 49140 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Covalent-bonded immobilization of enzyme on hydrophilic polymer covering magnetic nanogels
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی کاتالیزور
پیش نمایش صفحه اول مقاله
Covalent-bonded immobilization of enzyme on hydrophilic polymer covering magnetic nanogels
چکیده انگلیسی

A new mesoporous support, hydrophilic polymer covering magnetic nanogels containing reactive carboxyl groups, was obtained by photochemical in situ polymerization. The enzyme α-chymotrypsin (CT), a model for protein or esters hydrolysis, was anchored to the support by 1-ethyl-3-(3-dimethylaminepropyl) carbodiimide (EDC) activation. The immobilization proof was confirmed with Fourier transform spectroscopy (FT-IR) and transmission electron microscopy (TEM) observation. The bound CT behaved superparamagnetic and exhibited excellent response to applied magnetic field. The immobilized CT had better resistance to temperature and pH inactivation in comparison to the free form, and hence widened the reaction pH and temperature regions. Thermostability and storage stability of the enzyme increased up on covalent immobilization. The immobilized enzyme retained a relative activity of 85% after 12 repeated uses. Kinetic parameters were assayed for both free and immobilized CT. The value of Km (5.3 × 10−4 M) for the immobilized enzyme was larger than that of the free counterpart (3.7 × 10−4 M), while the Vmax (6.1 U mg−1 enzyme) was smaller for the immobilized enzyme.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microporous and Mesoporous Materials - Volume 109, Issues 1–3, 1 March 2008, Pages 470–477
نویسندگان
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