Données récentes sur la production d'anticorps monoclonaux à visée diagnostique en histopathologie

Since their invention in the seventies, the techniques for generating monoclonal antibodies in mice are constantly improving. The first antibodies applied for diagnosis purposes were first used for immunoblotting techniques. Later antibodies technologies were transferred on tissues sections with immunofluoresence and immunohistochemistry. It turned out that monoclonal antibodies were much more efficient than polyclonal ones, in particular on tissue sections. Beside their use in diagnostic pathology, monoclonal antibodies have been humanized to use them for treatment of several diseases such as cancers and inflammatory disorders. Monoclonal antibodies remain the best tools for diagnosis in routine histopathology. The recent production of rabbit monoclonal antibodies represents an important breakthrough in the field of immunohistochemistry.