Synthesis, molecular docking and biological evaluation of new steroidal 4H-pyrans

A convenient synthesis of new series of steroidal 4H-pyrans has been performed. After characterization, the interaction of compounds 4 and 5 with DNA was evaluated by gel electrophoresis, docking studies and UV-vis and fluorescence spectroscopy. MTT assay and comet assay has been performed to check the in vitro cytotoxicity of new compounds.