کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7674454 | 1495701 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Total reflection X-ray fluorescence measurements of S and P in proteins using a vacuum chamber specially designed for low Z elements
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Total reflection X-ray fluorescence measurements of S and P in proteins using a vacuum chamber specially designed for low Z elements Total reflection X-ray fluorescence measurements of S and P in proteins using a vacuum chamber specially designed for low Z elements](/preview/png/7674454.png)
چکیده انگلیسی
As the ratio of phosphorus and sulfur in proteins allows the determination of the phosphorylation degree in proteins, the absolute determination of phosphorus and sulfur in organic samples is of growing interest. While it takes some effort to quantify phosphorus and sulfur with inductively coupled quadrupole plasma mass spectrometry (ICP-QMS), total reflection X-ray fluorescence analysis (TXRF) allows easy quantification. In the presented work, the low Z TXRF spectrometer at the Atominstitut was used to analyze phosphorus and sulfur in proteins. Although the preparation of the protein samples proved to be more difficult than originally expected, it could be shown that TXRF is well suited for the determination of P and S in proteins. The obtained lower limits of detection (LLD) for P and S in proteins were extrapolated for 1000s and were 34Â pg and 19Â pg, respectively. The importance of height scans for each sample to exclude heterogeneities was demonstrated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Spectrochimica Acta Part B: Atomic Spectroscopy - Volume 101, 1 November 2014, Pages 118-122
Journal: Spectrochimica Acta Part B: Atomic Spectroscopy - Volume 101, 1 November 2014, Pages 118-122
نویسندگان
M. Rauwolf, C. Vanhoof, K. Tirez, E. Maes, D. Ingerle, P. Wobrauschek, C. Streli,