DNase I enzyme-aided fluorescence signal amplification based on graphene oxide-DNA aptamer interactions for colorectal cancer exosome detection

We established a DNase I enzyme-aided fluorescence amplification method for colorectal cancer exosome detection based on graphene oxide (GO)-DNA aptamers interaction, with good sensitivity and simple operation. The limit of detection of this method for colorectal cancer exosome is 2.1 × 104 particles/μl. This method was also verified in 19 clinical blood serum samples (5 μl) to distinguish healthy and colorectal cancer patients, which showed significant diagnostic power.175