کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7677545 | 1495775 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Selective extraction of endogenous peptides from human serum with magnetic mesoporous carbon composites
ترجمه فارسی عنوان
استخراج انتخابی پپتیدهای اندوژن از سرم انسان با کامپوزیت کربن مزوپور مغناطیسی
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کلمات کلیدی
ماده کربن استوانه ای، مغناطیسی، پپتیدهای اندوژن سرم انسان،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Highly sensitive and selective enrichment of endogenous peptides or proteins from complex bio-system takes a significant important place to the proteomic. In this work, a unique Fe3O4@2SiO2@mSiO2-C nanomaterial was synthesized, contributing to the separation and enrichment of low concentration peptides from complex mixture. The highly ordered mesoporous carbon structure render the nanospheres with unique properties of strongly connected pore channels, strong hydrophobic properties, high specific surface area (254.90Â m2/g), uniform pore size (3.61Â nm). Which made it a promising candidates for the efficient enrichment of peptides through hydrophobic-hydrophobic interaction with low detection limit (0.2Â fmol), superb size-exclusion of high molecular weight proteins, highly selectivity for BSA digest (molar ratio of BSA tryptic digests/BSA, 1:400), ideal peptides recovery (about 87.5%), wonderful repeatability (RSD less than 25%). Moreover, the as-prepared Fe3O4@2SiO2@mSiO2-C nanoparticles were successfully enriched 2198 endogenous peptides from human serum, which fully indicated that the mesoporous carbon nanoparticles was a promising candidate for isolating proteins or peptides from complex biologicals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 161, 1 December 2016, Pages 647-654
Journal: Talanta - Volume 161, 1 December 2016, Pages 647-654
نویسندگان
Yangyang Gan, Quanqing Zhang, Yajing Chen, Yiman Zhao, Zhichao Xiong, Lingyi Zhang, Weibing Zhang,