کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7678560 | 1495791 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An aqueous platinum nanotube based fluorescent immuno-assay for porcine reproductive and respiratory syndrome virus detection
ترجمه فارسی عنوان
یک آزمایش ایمونولوژیک فلورسنت مبتنی بر نانولوله های آب پزیزی برای تشخیص ویروس سندرم تولید مثل و تنفسی
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Porcine reproductive and respiratory syndrome virus (PRRSV) has been a significant pathogen towards global swine industry upon its emergence in the late 1980s and since then has exemplified a rapidly evolving, widely spreading pathogen. It is urgently important to develop a simple, rapid and cost effective method to detect this pathogen when virus outbreaks. In the present work, it was found that virus antibody modified platinum nanotubes (Pt-Ab) could act as a superquencher to CdTe:Zn2+ quantum dots (CdTe:Zn2+ QDs) fluorescence by Stern-Volmer constants nearly 109Â Mâ1 without any aggregation, the CdTe:Zn2+ QDs fluorescence will recover as the Pt-Ab goes away by antibody and antigen interaction when virus was added into the probe solution, releasing CdTe:Zn2+ QDs from the surface of Pt-Ab. By the recovery fluorescence intensity, it can realize qualitative and quantitative detection of PRRSV. This method gives a fast response to PRRSV concentration and provides a sensitive detection limit (2.4Â ng/mL). Moreover, it can be applied in infected porcine serum samples and obtain satisfied results.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 144, 1 November 2015, Pages 324-328
Journal: Talanta - Volume 144, 1 November 2015, Pages 324-328
نویسندگان
Lu Chen, Shiyi Ye, Kai Cai, Cuiling Zhang, Guohua Zhou, Zhike He, Heyou Han,