کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7678676 | 1495791 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A disposable electrochemical sensor based on protein G for High-Density Lipoprotein (HDL) detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A disposable electrochemical sensor based on protein G for High-Density Lipoprotein (HDL) detection A disposable electrochemical sensor based on protein G for High-Density Lipoprotein (HDL) detection](/preview/png/7678676.png)
چکیده انگلیسی
In this work, two biosensors were developed for the detection of High-Density Lipoproteins (HDL) particles, which are biomarkers inversely correlated with cardiovascular risk and which represent therapeutic targets for atherosclerosis. The electrochemical properties of the grafted antibody on interdigitated gold electrode were achieved by Impedance Spectroscopy (IS). The used deposition method was based on oriented antibody Anti-ApoA1 with an intermediate thin layer of protein G. The developed biosensor was able to detect both native plasma HDL and reconstituted HDL (rHDL) particles respectively with the detection limit of 50Â ng/mL and 1Â ng/mL, respectively. Dynamic contact angle and atomic force microscopy were used. The developed biosensors are able to differentiate the HDL particles according to their differences in size and interactions with the immobilized antibody.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 144, 1 November 2015, Pages 466-473
Journal: Talanta - Volume 144, 1 November 2015, Pages 466-473
نویسندگان
H. Chammem, I. Hafaid, N. Bohli, A. Garcia, O. Meilhac, A. Abdelghani, L. Mora,