کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7681825 | 1495818 | 2013 | 39 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
RNA aptasensor for rapid detection of natively folded type A botulinum neurotoxin
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A surface plasmon resonance based RNA aptasensor for rapid detection of natively folded type A botulinum neurotoxin is reported. Using detoxified recombinant type A botulinum neurotoxin as the surrogate, the aptasensor detects active toxin within 90Â min. The detection limit of the aptasensor in phosphate buffered saline, carrot juice, and fat free milk is 5.8Â ng/ml, 20.3Â ng/ml and 23.4Â ng/ml, respectively, while that in 5-fold diluted human serum is 22.5Â ng/ml. Recovery of toxin from disparate sample matrices are within 91-116%. Most significant is the ability of this aptasensor to effectively differentiate the natively folded toxin from denatured, inactive toxin, which is important for homeland security surveillance and threat assessment. The aptasensor is stable for more than 30 days and over 400 injections/regeneration cycles. Such an aptasensor holds great promise for rapid detection of active botulinum neurotoxin for field surveillance due to its robustness, stability and reusability.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 117, 15 December 2013, Pages 273-280
Journal: Talanta - Volume 117, 15 December 2013, Pages 273-280
نویسندگان
Pavithra Janardhanan, Charlene M. Mello, Bal Ram Singh, Jianlong Lou, James D. Marks, Shuowei Cai,