Development of diagnostic methods and study of the immunoreactivity of a mixture of recombinant core and E2 proteins fused to GST with control serum positive for hepatitis C

► Captured monoclonal anti-GST antibody ELISA gave greater specificity. ► Indirect ELISA immunoreactivity is increased by mixing recombinant proteins HCV, core and E2. ► Immunoblotting innovative method using the protein mixture HCV-core and E2. ► HCV diagnostic methods with high specificity and sensitivity.