Selection and identification of streptomycin-specific single-stranded DNA aptamers and the application in the detection of streptomycin in honey

► Sixteen ssDNA aptamers specific to streptomycin were screened and identified. ► The structures and the dissociation constants of the aptamers were characterized. ► STR1 was used in the detection of streptomycin in both solution and honey samples. ► The sensitivity, specificity and repeatability of the assay were studied.