A self-assemble aptamer fragment/target complex based high-throughput colorimetric aptasensor using enzyme linked aptamer assay

► High-throughput colorimetric aptasensor based on ELAA is established. ► Novel ELAA type using only a single aptamer was introduced into microplate. ► Detection is based on self-assembling aptamer fragment/target complex. ► The model is successfully used to detect cocaine and thrombin. ► It is a powerful platform to detect different kinds of targets.